ABSTRACT
Background: The potential involvement of Notch signaling pathway in cell fate decision, tumor heterogeneity and angiogenesis in solid tumors can be explored in colorectal cancer (CRC). This might further help to improve outcomes in CRC. Here, the promoter methylation of Notch receptor gene (Notch2 and Notch3) and their co-expression with its downstream transcription factor Hes1 has been analyzed. Methods: Seventy-two CRC patients were enrolled to study the role of Notch2, Notch3 and Hes1 genes in colorectal cancer. Promoter methylation and mRNA expression in tumor and adjoining normal tissue were assessed by Methylation Specific PCR and quantitative Real time PCR respectively. Statistical correlation was done by using SPSS. Results: We found that Notch2 and Notch3 were hypomethylated in 52/72 (72.22%) and 54/72 (75%) cases respectively. Hypomethylation of Notch 2 and Notch 3 showed significant association with advanced stage (p=0.001) and (p=0.003) and nodal metastasis (p=0.036) and (p=0.012) respectively. Both Notch 2 and Notch 3 showed increased mRNA expression in 49 (68.05%) and 51(70.84%) patients with a fold change of 3.37 and 5.43 respectively. Positive correlation between hypomethylation and expression was observed for both genes. High expression of Hes1 was found in 53(73.61%) of cases which was highly relatable with over expression of notch receptor genes. Upregulation of Notch 2, Notch 3 and Hes1 showed significant association with high grade tumors, advance stage and presence of LN metastasis, additionally Notch 3 and Hes1 showed significant association with distant metastasis. Conclusion: Hypomethylation of Notch 2 and 3 receptors is playing crucial role in regulating the expression of these genes in CRC. Overexpression of Notch 2, Notch 3 and Hes1 are associated with disease progression in CRC.
ABSTRACT
Background: Multi Detector computed tomography (MDCT) may effectively characterize and quantify the extent of emphysema and the air trapping related to the small airway’s disease. Here we highlight the computed-tomography findings of Chronic Obstructive Pulmonary Disease (COPD) and correlation with the Spirometrics values.Methods: The study group included the total of 100 adult patients of either sex with a clinical suspicion of COPD and those who undergone MDCT of thorax. Lung function of the patients with the COPD stages mild to very severe was evaluated by both the MDCT and Spirometrics Pulmonary Function Tests (PFTs). The scanning was done at maximum end inspiration and maximum end expiration.Results: There was a preponderance of male patients with highly significant correlation between values of mean lung density and low attenuation values (p<0.000I). MDCT correlated well with those obtained from spirometric Pulmonary Function Tests in the patients with COPD and that the correlation at expiration was superior to that at inspiration.Conclusions: The study concludes that Multi-detector computed tomography is the invaluable tool in defining and quantifying COPD and the characterization of emphysematous changes.